Inspired by a recent celebration of the work of Martin and Synge, this article examines the role of chromatography in efforts to elucidate the primary structures of proteins. It touches on, as I have referred to it, ‘the other Sanger sequencing’ – the method by which the first protein sequence was determined. It goes on to highlight some recent work on the application of a displacement method to the separation of peptides (Kwiatkowski et al., Anal. Chem. 2018, 90, 9951-9958).

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Contributed by George Preston

George has a Ph.D. from the University of Leeds, and for six years was a Research Associate at King’s College London.