Two Offers of Master Internships in IBMM (Montpellier, France)

Master/3e année ingénieur research internship Février- Juillet 2023

Charge-Transfer Templated Ligation of Peptide Bioconjugates

 

Period: 6 months from January/February to June/July 2023, 600 euros/months
Internship tutors: Pr. Gilles Subra IBMM, Dr. Muriel Amblard [email protected], [email protected] in close collaboration with the IBMM Oligonucleotide team (F. Morvan, M. Smietana).
Internship locations: IBMM, Pole Chimie Balard, Montpellier, Peptides www.ibmmpeptide.com Team

Summary
This Master’s research project in chemobiology is part of the ANR CHAMELEON project, which aims to develop an innovative, traceless and universal ligation strategy to generate DNA-DNA, peptide-DNA and peptide-peptide conjugates, at low concentrations in water. Our strategy is based on charge transfer interactions between the electron-rich dialkoxynaphthalene (DAN) donor and the electron-deficient naphthalene-tetracarboxyliquediimide (NDI). The Master’s internship will be dedicated to the development of Peptide-Peptide conjugates. Another internship is offered in the IBMM oligonucleotide team for the synthesis of DNA-DNA conjugates. Depending on the achievements of the Master, a PhD funding may be proposed.

Student work
The synthesis and supramolecular assembly of novel phosphodiester-bound water-soluble polyDAN and polyNDI motifs has recently been developed by the IBMM Oligonucleotide team. Based on this expertise, these tags will be covalently linked via a peptide cleavable linker to induce directed ligations in an aqueous medium. The Master student will carry out the synthesis, analysis and purification of peptide precursors using support synthesis methods. He or she will synthesize the conjugates with polyDAN or polyNDI and evaluate their introduction via an acido labile group of type Hmb. He or she will characterize the biomolecules by LC/MS and NMR (H and P). Finally, he will perform fragment coupling or cyclization tests and study the influence of concentration to evaluate the Charge-Transfer Templated Ligation recognition effect of polyDNA and polyNDI.

Competencies required
1. Synthesis of peptides, synthesis on support
2. Ligation
3. Characterization of biomolecules (LC/MS, NMR).

Required skills and soft skills
1. Scientific English, Organic Chemistry and Analytical Chemistry Master’s level.
2. Autonomy, scientific curiosity
3. Rigor, capacity for work
4. Good interpersonal skills, ability to report

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Master/3rd year engineer RESEARCH INTERNSHIP
February- July 2023

Peptide-Apatite hybrid nanoparticles for the treatment of complex wounds

 

Period: 6 months from January/February to June/July 2023
Internship tutors: Pr. Gilles Subra IBMM, Dr. Christophe Drouet CIRIMAT
Contacts : [email protected], [email protected]
Internship locations: IBMM, Pole Chimie Balard, Montpellier, Peptides www.ibmmpeptide.com and CIRIMAT team, Toulouse, PPB team
The work will take place mainly at the IBMM in Montpellier, with one or more short stays (5 days) in Toulouse at CIRIMAT for the synthesis and characterization of particles.

Summary
This Master’s research project at the interface of inorganic chemistry, organic chemistry and biology, aims to synthesize, characterize and evaluate bioorganic-inorganic peptide-apatite hybrid colloidal nanoparticles from a bioactive peptide regulating inflammation and promoting wound healing. This internship is part of a larger project, conducted in collaboration between IBMM and CIRIMAT and Regentis Pharma for the development of a new generation of bioactive dressings to treat complex wounds with high infectious potential. In 2022, IBMM and CIRIMAT developed an original synthesis to obtain new submicron bio-inspired materials, which combine an apatite core, a natural constituent of bone, and a crown of bioactive peptides (e.g. proangiogenic or antibacterial). It will be necessary to adapt this synthesis to new bioactive peptides, to characterize and biologically evaluate the objects obtained.
Depending on the achievements of the project, CIFRE thesis funding may be proposed at the end of the Master’s internship.

Student work
The Master student will carry out the synthesis, analysis and purification of peptide precursors of interest (e.g. sequence patented by Regentis Pharma) and pseudopeptide (e.g. phophorylated and phosphonated peptides) using support synthesis methods.
He or she will characterize the biomolecules by LC/MS and NMR (H and P). He or she will perform the one-step synthesis of colloidal apatite-peptide hybrid particles and characterize them by TEM, DLS, DRX and FTIR, or even ATG. He or she will perform ligand quantification by multinucleus NMR (H, F and P). He or she will study the stability of particles under conditions close to physiological conditions, and the possible release of bioactive peptides.
Finally, depending on the progress of the project, he or she will be able to study the toxicity of these particles and their pro-healing effect on an in vitro cell model.

Competencies required
1. Project management with several laboratories, work at the interfaces of disciplines.
2. Synthesis of peptides, synthesis on support, synthesis of phosphocalcic derivatives, characterization of nanoparticles (TEM, DRX, FTIR), characterization of biomolecules (LC/MS, NMR).

Required skills and soft skills
1. Scientific English, Organic Chemistry and Analytical Chemistry Master’s level.
2. Knowledge of biology and biochemistry will be appreciated
3. Autonomy, scientific curiosity
4. Rigor, capacity for work
5. Good interpersonal skills, ability to report

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Internship apatite peptide EN
Title: Internship apatite peptide EN (43 clicks)
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Internship chameleon EPS
Title: Internship chameleon EPS (38 clicks)
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